Astragalus Membranaceus 뿌리에서 Astragalus Polysaccharides를 추출하는 방법은?

신의 남편&에서 처음 언급된 아스트라갈로스#39;s Classic 의the Materia Medica under the name “Huangqi”, has a medicinal history of more than 2,000 years 그리고enjoys the reputation of being “the best Qi tonic”다다. It is a dual-use species of medicine 그리고food announced by the National Health Commission. Astragaluspolysaccharide is one of the main active ingredients of astragalus. It has various physiological functions such as improving immunity, 항균, anticancer, protecting the intestines, anti-aging, antiviral, and protecting the cardiovascular system[1-2]. It is also a natural medicine for the treatment of metabolic dysfunction-related fatty liver disease[3], and has become a hotspot of research in recent years. This paper reviews the literature on the extraction 방법아스트라갈루스 다당류의 and their application in health drinks, with the aim of providing guidance for the large-scale industrial production of astragalus polysaccharides and providing a reference for the development and utilization of astragalus polysaccharides and related products.

1 추출방법

그 발전 및utilization of astragalus조직 내 아스트라갈루스 다당류의 추출 효율과 밀접한 관련이 있다.아스트라갈루스 다당류의 추출 효율을 높이기 위해 학자들은 아스트라갈루스 다당류의 추출 방법에 대해 많은 연구를 수행해 왔다.아스트라갈루스 다당류를 추출하는 방법에는 여러 가지가 있다.일반적으로 사용되는 전통적인 추출 방법에는 물 추출과 알코올 강수법, 알칼리 추출과 알코올 강수법 등이 있다.최근에는 초음파보조추출, 마이크로파보조추출, 효소추출과 같은 현대적 추출방법도 널리 사용되고 있으며 실험결과 높은 추출효과가 있음을 확인하였다.

1.1 물 알코올 강수량

The traditional water-alcohol precipitation method uses hot water to extract or boil the material to obtain an extract, which is then concentrated and precipitated to obtain an extract. This method is inexpensive and safe, and is currently the most commonly used method [4]. However, it consumes a lot of solvent, the purity of the polysaccharides is relatively low, and the difficulty of separation and purification increases. In order to improve the yield of polysaccharides, many researchers have carried out research on the extraction process. Wen Xiyan et al. [5] used the yield of dry extract and the polysaccharide content as evaluation indicators to explore the 효과of the liquid-to-material ratio, extraction time and number of extractions on the efficiency of 다당류 추출.

The results showed that the number of extractions and time had a significant effect on the efficiency of polysaccharide extraction, while the liquid-to-material ratio was not statistically significant. The results of the orthogonal test showed that the highest yield of extract was obtained when the liquid-to-material ratio was 1:8, the extraction was carried out three times, and each extraction lasted 1.5 h. Qiao Lijie et al. [6] used Tianshan Astragalusas the research object and determined the polysaccharide extraction process through comprehensive analysis of response surface and single factor: a liquid-to-material ratio of 1:32 and extraction at 92 °Cfor 3 h. Practical verification has shown that the polysaccharide content obtained using this process is close to the predicted value.

알칼리 추출과 알코올 강수량 1.2

Compared with water extraction and alcohol precipitation, it has the obvious advantages of high purity and stability of the product, but it has high requirements for equipment in practical applications, and the wastewater generated during the extraction process needs to be treated before it can be discharged.

Jin Fenfen et al. [7] investigated the effect of different extraction methods on the extraction rate of astragalus polysaccharides. The results showed that the polysaccharide extraction rate was 9.74% when extracted with an alcohol solution with a concentration of 5% and a pH of 12, while the polysaccharide extraction rate was 7.64% when extracted with alkali water alone. It can be seen that the alkali-alcohol extraction method is superior to the alkali water extraction method. Xie Dandan et al. [8] used a 1% KOH solution to extract astragalus polysaccharides, comparing the effects of different extraction temperatures, extraction times and material-to-liquid ratios on the polysaccharide extraction rate. The results showed that the optimal process for the alkaline extraction and alcohol precipitation method was a material-to-liquid ratio of 1:20, and the extraction was carried out at 70 °C for 130 minutes.

초음파를 이용한 추출 기술 1.3

이 방법은 초음파의 캐비테이션 (cavitation) 효과를 이용하여 추출 용매가 세포 내로 들어가게 하여 활성 성분의 방출과 용해를 가속화시킨다.이 방법은 조작이 간단하고, 추출온도, 추출시간, 고체-액비, 초음파동력 등이 주요 공정변수이다.첸쇼니 [9] 가 사용했다Astragalus membranaceus processed pieces as the raw material and, by optimizing the extraction process, achieved a polysaccharide yield of 5.862%. Wei Zengyun[10], Wang Dan[11], Li Lihong[12] and other researchers used the orthogonal array method and response surface method to optimize the extraction process.

유평 [13]은이 두 최적화 방법을 비교했다.연구 결과, 직교배열법에 비해 반응표면법에 의해 구축된 최적화 모형이 여러 요인 수준에서 추출률을 더 잘 예측할 수 있으며, 정확도도 약간 높은 것으로 나타났다.따라서의 공정 최적화를 위해 반응표면법을 적용하였다아스트라갈로사이드 IV 추출최적의 공정 조건을 선별하기 위해서.본 논문은 중국 국가지식인프라의 문헌을 검토하여 다양한 연구자들의 추출과정을 < 표 1 >과 같이 다음과 같이 요약한다.초음파를 이용한 추출 기술이 제어가 쉽다는 연구 결과가 다수 발표됐다.전통적인 추출 방법과 비교하여 빠르고 저온이며 에너지 절약적이고 환경 친화적이며 아스트라갈로사이드 IV의 산업 생산에 사용될 수 있습니다.

1.4 마이크로파 보조 추출 방법

This method uses the thermal effect of microwaves to cause cracks or pores in the plant cell wall, facilitating the dissolution of intracellular substances. It has the advantages of being easy to operate, fast, low cost, high efficiency, few by-products, and easy extraction, but there is a possibility that it may affect the structure and physiological activity of polysaccharides. In recent years, it has been widely used in the field of extracting the traditional Chinese medicine의 활성 성분.

Factors affecting this method mainly include microwave power and extraction time. Prolonging the extraction time can increase the reflection and absorption of microwaves, enhance the penetrating effect of microwaves on astragalus, and improve the extraction rate of polysaccharides [15]. Liang Taishuai et al. [16] studied the extraction conditions of astragalus polysaccharides 에서Astragalus membranaceus by orthogonal design. The results showed that the highest polysaccharide yield was 7.97% with a liquid-to-solid ratio of 20:1, microwave extraction for 10 minutes at a power of 450 W, and a total of 3 extractions. Zhao Fengchun et al. [17] studied the optimal process conditions to be an extraction time of 10 min, a microwave power of 360 W, and a liquid-to-solid ratio of 5:1, with a polysaccharide yield of 3.28%. Compared with direct heating extraction, the extraction time can be significantly shortened, but the purity is low.

효소 가수분해 1.5

This method uses enzymes such as cellulase and pectinase to break down the cell walls and promote the release of intracellular polysaccharides. Long Liangkun et al. [18] selected cellulase to hydrolyze the Astragalus membranaceus residue, and used single factor experiments and orthogonal experiments to explore the effects of enzyme dosage, temperature and time on polysaccharide extraction and to optimize the extraction process. The results showed that : When the amount of cellulase enzyme used is 4 U/g and the enzyme is hydrolyzed at 45 °C for 4 h, 44.6 mg/g polysaccharides can be obtained, which is 41.7 mg/g higher than the control group. It is clear that the use of cellulase to enzymolyze astragalus can greatly increase the amount of polysaccharide extracted. Dong Lingling et al. [19] used the extraction rate and mass fraction of astragalus polysaccharide as the indicators for investigation, and used the cellulase enzymolysis-microwave extraction method to extract astragalus polysaccharide, and compared the effects of different extraction methods.

The results showed that: at an enzyme-to-material ratio of 57.6 U/g, a liquid-to-solid ratio of 10:1, and an enzymatic hydrolysis time of 60 min, the highest polysaccharide extraction rate of 16.07% was achieved using microwave power of 480 W, and the polysaccharide mass fraction was 88.40%. The extraction rates of the direct enzymatic method, microwave extraction method, and water reflux extraction method were 10.64%, 13.74%, and 4.82%, respectively, and the mass fractions of the three were 63.86%, 66.00%, and 37.54%, respectively. It can be seen that the enzymatic hydrolysis-microwave extraction method has the highest polysaccharide extraction rate and quality fraction among the four methods. This method has the advantages of short extraction time, mild enzymatic hydrolysis conditions, low energy consumption, and extracts that are not easily damaged. It is a good method for extracting active ingredients 에서plant cells.

건강음료의 아스트라갈루스 다당류 적용 현황 2

2.1 아스트라갈루스 건강음료

With increasingly fierce social competition and faster and faster work rhythms, fatigue has become a common phenomenon. Health drinks that relieve fatigue, restore physical strength, and improve the body'의 저항이 연구의 열점이되었다.서린 등 [20]은 아스트라갈루스와 메들라를 원료로 사용하여 소르비톨, 키위즙, 잔탄검 등을 농축하여 아스트라갈루스 고유의 풍미를 가진 아스트라갈루스 메들라 복합 음료를 제조하였다.마오우징 [21]은 행산, 대추, 루당 인삼의 Astragalus membranaceus를 2:2:1 비율로 원료로 사용하였다.추출 및 제조 공정을 최적화하여 과립과 분말 두 종류의 피로 방지 고체 음료를 얻을 수 있었다.포뮬러는 피로 억제 효과가 큰 것으로 나타났다.많은 학자들이 아스트라갈루스 다당류의 노화 방지 효과를 연구하여 복합 아스트라갈루스 노화 방지 음료, 밤 아스트라갈루스 산화 방지 음료, 아스트라갈루스 장미 산화 방지 및 주근깨 제거 음료 등을 개발했다.이러한 건강음료의 개발은 아스트라갈루스와 그 추출물의 개발과 응용에 새로운 길을 제공하였다.

아스트라갈루스 건강 요구르트 2.2

Astragalus juice has a certain antibacterial effect. Wang Dan [22] used an ultrasonic extraction method to obtain an astragalus extract, mixed it with skim milk powder and sucrose in proportion, homogenized it, added Lactobacillus bulgaricus and Streptococcus thermophilus to ferment it to obtain a health yogurt, and studied its fermentation process. The optimal fermentation process determined by response surface was: the addition of astragalus polysaccharide was 5%, the addition of sucrose was 10%, and the fermentation time was 6 h. Under these conditions, the yogurt had a good flavor and the sensory evaluation score was the highest, 88 points. Zhang Rong [23] developed a yogurt with significant hypoglycemic activity from Astragalus. The product contains 15.25% Astragalus juice, 8% maltitol, and 1.93% xylo-oligosaccharides. was 1.93%. A mixed culture of Streptococcus thermophilus, Lactobacillus bulgaricus and Bifidobacterium adolescentis was used in equal proportions, with an inoculum of 3.53% and fermentation at 43°C for 5 hours. Under these fermentation conditions, the sensory score was the highest, at 94.4 points. Studies have shown that it also has a beneficial effect on kidney and blood lipid damage.

아스트라갈루스 건강차 2.3

Astragalus tea is a major health tea variety with many benefits, including delaying aging, regulating blood sugar, improving myocardial blood supply, and boosting the immune system. Astragalus tea is easy to make, just brew with boiling water, and it is convenient to drink. There are different types, such as those made with a single herb or a blend of herbs. Cheng Likun et al. [24] developed an eight-treasure tea using astragalus, ground-cherry, black medlar, jujube, raisin, etc. as raw materials. This tea has the effect of nourishing the qi and replenishing blood, relieving summer heat and fire, and is very suitable for nursing mothers. At present, the production of health tea is relatively less mechanized, and there is still a gap between it and the requirements of standardization and industrialization. It should be actively explored to achieve the in-depth sustainable development of the health tea industry.

아스트라갈루스 헬스 와인 2.4

Many scholars have used the soaking and blending process or the preparation process of distilled wine to develop astragalus health wine, and have achieved a series of research and development results. For example, Chai Zhi et al. [25] used sorghum and astragalus as raw materials, mixed them in a ratio of 1:9, fermented at 15 °C for 40 h, distilled, and blended to obtain a fermented distilled liquor containing astragalus total polysaccharides. Yang Lihua et al. [26] used yellow rice and glutinous rice as raw materials, added Astragalus membranaceus water extract for fermentation, and obtained Astragalus membranaceus rice wine containing polysaccharides. Feng Ziyao et al. [27] used apple pomace and chestnut residue produced during enterprise production as raw materials for enzymatic hydrolysis, fermentation and distillation, mixed them with Astragalus extract in proportion to prepare Astragalus formulated wine, which has low production cost, solves enterprise problems and provides new ideas for the development of health foods.

3 전망

There are many methods for extracting astragalus polysaccharides, and each method is imperfect, with its own advantages and disadvantages. Therefore, when choosing a specific method, factors such as extraction rate, difficulty of separation and purification, polysaccharide activity, equipment conditions, and extraction cost should be comprehensively considered. Studies have found that the yield of astragalus polysaccharides extracted using similar or the same method also varies greatly. Extraction conditions such as extraction time and temperature have a significant impact on the yield of polysaccharides. Therefore, after selecting a method, the extraction process should be optimized to achieve the maximum extraction rate.

The extraction of astragalus polysaccharides using the cellulase method has a high yield and stable product, and the reaction conditions are mild, energy-saving and environmentally friendly. It is a better method for extracting plant polysaccharides. It is recommended that further research be conducted to determine the optimal extraction process and formulate extraction specifications, so that this method can be better applied in large-scale industrial production.

In recent years, scholars have combined two or more modern extraction methods such as enzymatic extraction, microwave extraction, and ultrasonic extraction to extract astragaloside, which yields higher yields than a single extraction method. The monosaccharide composition and glycan structure of astragalus polysaccharides obtained by different extraction and purification methods are different, but the effects of modern extraction methods on the structural changes, stability, physiological activity and clinical efficacy of astragalus polysaccharides are still unclear.

It is recommended to strengthen in-depth research to continuously improve the extraction and purification methods to prepare high-efficiency and high-quality astragalus polysaccharides, and provide raw materials for the development of astragalus polysaccharides and related products.

A large number of studies have shown that astragalus polysaccharides have obvious physiological activities such as anti-oxidation, antibacterial, immunity enhancement, and anti-tumor. After being extracted according to the methods described in the article, they are used in health foods, and the market prospects are broad. At present, most research on astragalus health drinks focuses on new product development and process optimization, with insufficient evaluation of product functionality or testing of functional ingredients. This should be given attention in order to better promote the application of astragalus health products.

참조:

[1]   첸 R Z, 황갈색 L, 진, C  G,et al. 다당류의 추출, 분리, 특성 및 항산화 활성 from   Astragalus   membranaceus다 [J다]산업  농작물  and  제품, 2015년 77:434-443다.

[2] Liu Ying, Zhang Jinlian, Deng Yaling 외.아스트라갈루스 다당류의 추출, 분리 및 정제와 그 약리학적 효과에 대한 연구 진행 [J.한의과학지 2021, 36 (10):6035-6038.

[3] ZHANG J,FENG Q S.Pharmacological effects and molecu ‐lar 보호 메커니즘 of  Astragalus 다당류  비알코올성 지방간 질환 [J.「 Frontiers in Pharma ‐ cology 」,2022,13:854674.

[4]   SHANG H M,WANG M H,LI R,et. 추출 조건 최적화 and  effects  의 건조 methods  polysac ‐ charides의 physico ‐ chemical properties와 항산화 활성에 대한 자료입니다 from   Astragalus  cicer l. [J].Scientific 보고서, 2018,8(1):3359.

[5] 원시옌, 샤오징, 왕란시아 등.직교설계를 이용한 아스트라갈루스 다당류의 추출공정 선정과 세 가지 색상측정법 [J.대한한의학회지 2018년, 33 (4):1562-1566.

[6] 챠오 리지, 왕안핑, 아이샤 구올리 바스카 외.안산암으로부터 아스트라갈루스 다당류의 추출과정을 최적화하기 위한 반응표면법 (Response surface method) [J.한국식품안전품질시험학회지, 2020, 11 (1):59-65.

[7] 진 펜펜, 진 신쟈, 양희신 외.다양한 추출방법이 아스트라갈루스 다당류의 추출율에 미치는 영향에 관한 실험적 연구 (J.대한한의학회지 2013, 31 (10):2136-2138.

[8] 시단단, 정단, 장리하.astragaloside polysaccharide의 추출 및 항산화 활성 (J.천진농학, 2019, 25(12):19-23.

[9] 천슈니.아스트라갈루스 다당류의 추출공정 연구 (J.시젠한의학, 2014, 25(4):845-846.

[10] 위정연, 동팡주안, 보이란.반응표면 방법론을 이용한 아스트라갈루스 다당류의 초음파 보조 추출의 최적화.중국농업과학기술보고 2022, 24(4):203-209.

[11] 왕단, 왕후안.실험설계-반응표면방법론을 이용한 아스트라갈루스 다당류의 초음파 추출 최적화.Anhui Chemical Industry, 2019, 45(4):29-32.

[12] 리리홍, 신팅, 천종지 외.아스트라갈루스 다당류의 초음파 추출공정 최적화 및 다양한 기원의 아스트라갈루스의 비교연구 [J.한국정저우동물공학회지 2013, 33(2):1-3, 27.

[13] 유핑, 자오바오'e, Li Qian.아스트라갈루스 다당류의 다양한 추출 최적화 과정 [J.세계한의학, 2021, 16(12):1786-1791.

[14] 순펑갸오.모델링, 시뮬레이션 및 최적화 of ultrasonic coupling enzyme-assisted continuous countercurrent extract of astragalus polysaccharides [D].광저우:화남공과대학, 2019.

[15] BEN SALEM Y, ABDELHAMID A, MKADMINI 함미 K 외.Microwave-assisted 추출 및 pharmacologi cal evaluation of polysaccharides from Posidonia oceanica [J].「 생명공학, Biotechnology, and Biochemistry 」, 2017, 81 (10):1917-1925.

[16] Liang T, Zhang M, Jiang J. orthogonal test에 의한 Astragalus membranaceus 로부터 Astragalus 다당류의 마이크로파 보조 추출 최적화.중국의 전통 및 민간의학, 2022, 31(1):63-67.

[17] 자오펑춘, 장옌중.astragalus polysaccharide의 마이크로파 추출 공정에 대한 연구.[J]다.시젠국약, 2009, 20(4):912-913.

[18] 장량군, 한지춘, 린커잉 등.효소법에 의한 아스트라갈루스 잔류물로부터 다당류의 제조 및 항산화 활성 [J.장수농학, 2018, 46(1):137-140.

[19] 동링링, 황신, 키양광 등.효소가수분해법 (enzymatic hydrolysis-microwave method)에 의한 아스트라갈루스 다당류 추출공정에 관한 연구 (J.절강대학교 논문집 2011, 39(5):528-531.

[20] 서린, 위kongjiong, 장자오룽 등.Astragalus와 wolfberry 복합 음료의 연구 및 개발 [J.음료산업, 2020, 23(5):37-42.

[21] 마오우징.Astragalus membranaceus 항 피로 고체 음료 [D]의 연구 및 개발.타이위안:산시대학, 2020.

반응표면방법론을 이용한 아스트라갈루스 건강요구르트 공정의 최적화 연구 (22) Wang D.중국식품산업, 2019 (5):68-71.

[23] Zhang R. Research and development of Astragalus and Anemarrhena asphodeloides 요구르트 및 그 활성 [D].허페이:허페이 공과대학교, 2013.

[24] 청리쿤, 후루이춘, 양류 외.디바오바오차 [J]의 개발과 연구.「 내몽골대학학보 」, 2021, 40 (1):52-57.

[25] 차이즈, 장루난, 판후이지 등.발효와 증류 Astragalus membranaceus wine의 생산공정에 관한 연구 (J.중국양조, 2018, 37 (5):198-202.

[26] 양리화, 루나, 장수충 외.Astragalus membranaceus wine의 발효조건 최적화 (J.식품연구개발, 2022, 43 (8):106-111.

[27] 펑쯔야오, 장핑핑, 장캔 등.사과 포마스와 밤 포마스의 아스트라갈루스 와인의 가공기술에 관한 연구 (Research on the processing technology of astragalus wine from apple pomace and chestnut pomace)식품연구 개발, 2021, 42 (15):47-52.