흑마늘의 용도는?

Black garlic is made from fresh raw garlic, with the skin left on, and fermented in a fermentation chamber for 90 to 120 days다. It was first produced in Aomori Prefecture, Japan, and was introduced to China about 10 years ago, where it gradually became popular. 이re are many different types of black garlic, and it quickly captured the market. Black garlic is soft and shiny, with a soft texture and a sweet and sour taste that is not irritating. It not only removes the unpleasant flavor and irritation of garlic, but also adds a rich aroma that enhances the appetite. 이chemical composition of black garlic also undergoes significant changes compared to that of regular garlic, producing pharmacological effects that garlic does not have[1]. This article provides a review of the chemical composition, pharmacological effects, processing technology, and related product categories of black garlic, as well as an outlook on the future development and application of black garlic.

흑마늘의 화학성분 1

1. 1 Melanoidins

Black garlic melanoidins are a type of tan substance formed by the Maillard reaction of sugars and proteins, peptides, amino acids, etc. in garlic. They have similar specific surface areas, similar structures, and varying degrees of polymerization, and are one of the reasons why black garlic is black. Studies have shown that black garlic black essence mainly includes furans, pyrroles, thiophenes, phenols and alkanes, of which the furans have the highest content (more than 45%). Among the furans, 3-methylfuran, 2,5-dimethylfuran and 5-hydroxymethylfurfural have the highest content, which are mainly converted from reducing sugars in garlic[2]. Melanoidins have a very complex structure. The structural identification of melanoidins in black garlic shows that they have a backbone similar to sugar chains, and a relatively high proportion of sulfur, indirectly proving the possibility of participation of the amino acid S-methylmercaptopropionate in the Maillard reaction [3].

Melanoidins not only change the color and flavor of food, but also have antioxidant, prebiotic, and antihypertensive effects. Zhao Yimeng et al. [4] used high hydrostatic pressure to assist in the extraction of melanoidins from black garlic. The study showed that melanoidins are less stable under conditions such as the presence of an oxidant, ultraviolet light, and sunlight. Wang Yueli [5] showed that black garlic black essence extracted with ethanol and purified by ultrafiltration and gel permeation chromatography can effectively scavenge 1,1-diphenyl-2-trinitrophenylhydrazine free radicals and hydroxyl radicals. The relative molecular mass is directly proportional to the antioxidant capacity. Some studies have shown that the antioxidant capacity of black essence with decolorization treatment decreases significantly, which indirectly confirms the antioxidant effect of black essence pigments [6]. In addition, black garlic black essence can inhibit obesity by regulating the circadian rhythm of the intestinal flora in obese mice induced by a high-fat diet [7], and has broad application prospects in fat-reducing foods. However, the preparation process of black essence from black garlic is still immature, and there has been little research on its pharmacological effects and mechanisms. Research on its structure and production reactions is difficult.

단백질과 아미노산 1.2

During the fermentation process, nitrogen-containing compounds in garlic are converted into proteins, and proteins are partially broken down into amino acids. Black garlic is rich in 18 amino acids, including lysine, valine, and tryptophan, as well as eight essential amino acids for the human body [8]. Zheng Lan et al. [9] determined the amino acids by acid hydrolysis, and the results showed that most of the hydrolyzed amino acids increased significantly during processing, with the largest increases in alanine and leucine, while the contents of cysteine and arginine decreased significantly. Cysteine is the parent of black garlic flavor compounds and is also the main precursor of garlic sulfur compounds. The decrease in its content may be one of the reasons for the formation of black garlic flavor. Wang Congcong et al. [10] used the ninhydrin colorimetric method to study the changes in the content of total free amino acids during the processing of black garlic, and the results showed an initial increase and then a decrease. Yang Qingli et al. [11] also found that the content of most amino acids was inversely proportional to the processing time.

The results of studies on the changes in amino acid content after black garlic processing are not the same, and the results of protein-related studies also vary greatly. For different varieties of black garlic, the protein content of single-clove black garlic increased significantly before and after fermentation, while there was no significant change in multi-clove black garlic [12]. Niu Nana et al. [13] observed that the soluble protein content of black garlic continued to increase during processing. Zhang Zhenghai et al. [14] showed that the overall increase in crude protein was significant, while Sun Yue'e 등 15)의 연구결과 가공 후기에 단백질 함량이 점차 감소하였다.이는 흑마늘의 품종, 가공방법, 시료 크기 등과 관련이 있을 것이다.아미노산 및 단백질 함량의 감소는 Maillard 반응에 의한 섭취의 결과일 것으로 추측된다.아미노산과 단백질은 흑마늘의 중요한 영양소이다.현업을 바탕으로 흑마늘 품종과 가공기술을 최적화하면 흑마늘 제품 개발 및 응용에 도움이 될 것이다.

1. 3 폴리 페놀

Polyphenols are a general term for compounds in plant foods that have potential health-promoting effects. They generally have strong antioxidant effects and are commonly used as antioxidants and sunscreens. Garlic is one of the most important sources of polyphenols다.흑마늘로 가공한 후 폴리페놀 함량이 몇 배 증가할 수 있는데, 이는 거대분자물질이 가수분해되어 다량의 페놀성 히드록시기를 방출하고 다른 물질이 고온에서 전환되는 것과 관련이 있을 수 있다.항산화 능력은 그 함량에 정비례한다 [16].

The main components of black garlic polyphenols are flavonoids, phenolic acids and tannins. Current research focuses mainly on the extraction process and its antioxidant effect. Wu Ting et al. [17] prepared black garlic polyphenols using a microwave-assisted extraction method. Ethanol was used as the extraction solvent, with an extraction time of 12 min, the yield was only 8.66 mg/g; Huang Jiajia et al. [18] and Song Xiaohong et al. [19] used an extraction time of 40 min and 30 s, with yields of (6.15±0.50) mg/g and 1.885 mg/g, respectively; Zheng Qing et al. [20] used 60 % acetone instead, with an extraction time of 96 min, yield increased to 13.31 mg/g. Wang Zhao et al. [21] used a warm soaking method, using acidified ethanol as the extraction solvent based on the characteristics of phenolic hydroxyl groups. The extraction rate was 23.0037 mg/g for 60 min, and 20.4045 mg/g for the unacidified group, proving that acidification is beneficial to improving the extraction rate of black garlic polyphenols and enhancing its antioxidant capacity. In addition, Yu Zhongming et al. [22] used a composite enzyme method to extract black garlic polyphenols, and the extraction yield at 60 min was 8.087 mg/g. In summary, microwave-assisted extraction may not be suitable for the extraction of black garlic polyphenols. The extraction solvent and time have a significant effect on the extraction yield of black garlic polyphenols, and the advantage of the warm soaking method for extracting black garlic polyphenols is obvious.

1.4 유황 함유 화합물

Sulfur-containing compounds are the most active substances in garlic. After processing into black garlic, the total amount remains basically unchanged, and is about four times that of high-organic-sulfur-containing vegetables such as onions and cabbages. Black garlic contains 27 sulfur-containing compounds, of which the contents of alanine, deoxyglycine, and γ-glutamylcysteine are relatively high. Currently, the commonly used extraction methods include the fixed sulfur method, thin layer chromatography scanning method, high performance liquid chromatography-tandem mass spectrometry method and high performance liquid chromatography (HPLC) method. Among them, HPLC is currently the most widely used, efficient and economical method. Lu Lian-deng et al. [23] used HPLC to simultaneously determine the contents of alanine, deoxynojirimycin and γ-glutamylcysteine for the first time. The results showed that there were certain differences in the contents of the three sulfides in the seven commercially available black garlics.

Allyl sulfide is a non-protein sulfide amino acid unique to garlic, and has been developed into preparations such as oral solutions, pellets, and injections. Allyl sulfide has no bactericidal activity, but can bind to certain bacteria or bacterial enzyme proteins to block the exchange of substances between the bacteria and the outside world, and is partially converted to allicin during the process, enhancing the bacteriostatic effect [8]. Allyl sulfide can reduce reactive oxygen species (ROS) and mitochondrial membrane potential, inhibit the proliferation of gastric adenocarcinoma cells; and increase the activity of human salivary aldehyde dehydrogenase, which to some extent reduces the incidence of oral cancer; multiple studies have found that it can slow tumor progression by inhibiting or directly killing, and it is worth further research [24]. In addition, allicin also has anti-inflammatory, antioxidant, and immune-modulating functions. Other sulfides, such as S-allylmercaptocysteine (SAMC), have a good inhibitory effect on tumors; allyl sulfide has neuroprotective and antioxidant effects; and allicin can improve memory [25].

1. 5 설탕

One reason why black garlic is softer and sweeter than regular garlic is the increased content of water-soluble sugars. Black garlic can contain up to 60% water-soluble sugars, dozens of times more than regular garlic. The main types of sugars in black garlic are rhamnose, fructose, glucose, sucrose and maltose, with fructose being the most abundant and sucrose the least abundant [3].

Current research on the sugars in black garlic focuses on the polysaccharides. Studies have shown that black garlic polysaccharides can effectively scavenge free radicals, inhibit the increase in ROS caused by inflammation, and have significantly higher antioxidant activity than garlic polysaccharides but slightly lower than vitamin C. Black garlic polysaccharides can also inhibit energy metabolic disorders caused by free radicals and effectively resist fatigue [26]. Black garlic polysaccharide extract can significantly reduce the blood glucose concentration of mice with hyperglycemia without obvious toxic side effects [27]. In addition, black garlic polysaccharides can inhibit X-ray-induced genetic material changes in mice, and have a certain radiation protection effect [28]. Some studies have shown that garlic polysaccharides have obvious laxative, immunomodulatory, antiviral and other effects, which can be used as a reference for studying the pharmacological activities of black garlic polysaccharides.

흑마늘의 약리학적 효과 2

2. 1 항 산화

The antioxidant activity of black garlic is the pharmacological effect that has been studied the most and in the greatest depth in recent years. In terms of liver protection, studies have shown that aged 흑마늘 추출물 can significantly increase the content of glutathione and the activity of antioxidant enzymes in the liver, and significantly reduce the oxidative damage caused by long-term alcohol consumption to the DNA of blood lymphocytes [29]. A black garlic powder solution can increase the activities of superoxide dismutase (SOD), glutathione peroxidase and catalase in the livers of diabetic mice, and reduce the content of lipid peroxide malondialdehyde in the liver. It can also protect the liver and kidney cells of diabetic rats by restoring the levels of urea, creatinine, β2-microglobulin and total protein[30]. protect the liver and kidney cells of diabetic rats [30]. Tsai et al. [31] showed that the main components of the liver-protecting effect of black garlic may be S-allylcysteine (SAC) and black garlic polysaccharides, which inhibit CCl4-induced liver damage by inhibiting lipid peroxidation.

In terms of cardiovascular and cerebrovascular health, black garlic can reduce the risk of cardiovascular disease by inhibiting H2O2-induced endothelial cell damage [32]; 40% ethanol extract pretreatment of 2,2-azobis(2-methylpropionamidine) dihydrochloride-induced damage to red blood cells significantly reduced the hemolysis rate, showing strong intracellular antioxidant activity [33]. Black garlic can also increase the body's 항산화 수준, 관상 동맥 심장 질환 및 만성 심부전 환자의 n-말단 뇌 natriuretic 펩타이드 전구 함량을 줄이고 좌심실 박출 분율을 개선 [34];발스 등 [35]은 고콜레스테롤혈증 환자를 대상으로 한 임상시험에서 흑마늘 추출물을 6주간 복용한 후, subjects'확장기 혈압은 일반적으로 효과적으로 감소되었다.또한, 흑마늘 에탄올 추출물은 글루탐산나트륨으로 인한 작업기억력 장애에 대한 예방효과 (36;흑마늘 추출물의 알리신은 활성산소를 제거하고, 몸을 개선's 항산화 용량, 그리고 Alzheimer&의 학습 및 기억 능력을 향상시킵니다#39;s 병 모델 생쥐 [37];다른 연구에 의하면 흑마늘 나노밀이 흑마늘 추출물 [38]보다 쥐의 학습 및 기억력 향상에 더 좋은 효과가 있다고 한다.

The antioxidant mechanism of black garlic is currently unclear. Some studies have suggested that it is related to the upregulation of antioxidant enzymes that is dependent on the nuclear factor E2-related factor 2 (Nrf2) signaling pathway. Studies have shown that dithiobisallyl disulfide can induce an increase in Nrf2 protein levels, which in turn activates antioxidant enzymes [39]. Another study showed that black garlic may be able to block the oxidative stress induced by tert-butyl hydroperoxide in rat hepatocytes, which may be related to the regulation of the c-Jun amino-terminal kinase signaling pathway [40].

항균 및 항염증 2.2

Most of the sulphur compounds in black garlic have a significant antibacterial effect. It has been reported that the antibacterial effect mainly depends on allicin, which can kill more than 90% of saliva bacteria, especially methicillin-resistant Staphylococcus aureus [41]. Several studies have shown that black garlic combined with antibacterial drugs can effectively reduce the expression of virulence genes and reduce drug resistance. Nano-scale iron sulfide, which is converted from the sulfur compounds in black garlic, has been shown to have a broad-spectrum and potent bactericidal effect [42].

The main compounds in black garlic that exhibit anti-inflammatory effects are acetone, 2-linoleoyl-rac-glycerol and 5-hydroxymethyl furfural (5-HMF). 5-HMF is a product of black garlic processing, which exerts an anti-inflammatory effect on human umbilical vein endothelial cells stimulated with tumor necrosis factor-α by inhibiting the expression of vascular cell adhesion molecule 1, ROS and nuclear factor κB (NF-κB) activation [43]. In mouse monocytic leukemia cells, 5-HMF inhibits the production of prostaglandin E2 and pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) production, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, and NF-κB p65 nuclear translocation, as well as anti-inflammatory effects on the protein kinase B/mammalian target of rapamycin (Akt/mTOR) pathway in mouse monocytic macrophage leukemia cells [44]. Cui Xiaoyu et al. [45] used black garlic extract to intervene in a BALB/c mouse model of ulcerative colitis and similarly found that the concentrations of cytokines such as TNF-α, IL-1β and IL-6 were significantly reduced. At the same time, black garlic extract can reduce lung inflammation caused by sepsis by downregulating iNOS and COX-2. Male Wistar rats treated with black garlic extract for 20 consecutive days to treat gastric ulcers showed significantly lower levels of prostaglandin E2, glutathione and NO in the stomach [46]. Therefore, the anti-inflammatory mechanism of black garlic may be related to the direct inhibition of inflammatory-related transcription factors.

2. 3 Antitumor

Black garlic has a certain anti-tumor effect, and related research is still in its infancy. Shin et al. [47] showed that black garlic extract inhibited the growth of HT29 colon cancer cells by upregulating phosphodiesterase and tensin homolog genes, downregulating the expression of Akt and phosphorylated protein kinase B, and inhibiting the activity of their downstream target 70 kDa ribosomal protein S6 kinase 1 at the mRNA and protein levels. Duruishuo et al. [48] found that black garlic can significantly inhibit the proliferation of human liver cancer cells, induce apoptosis and have a cycle arrest effect, which may be related to the upregulation of Bcl-2-associated X protein, caspase-3, and downregulation of B-cell lymphoma/leukemia-2 protein. Black garlic extract can also inhibit the levels of matrix metalloproteinase-2, matrix metalloproteinase-9 and tight junction protein, thereby inhibiting the metastasis and invasion of gastric tumor cells [49]. Similar inhibitory properties have also been reported for black garlic extract and its compound SAMC in Lewis lung cancer cells and thyroid cancer cell lines, respectively. The anti-cancer effects of SAMC have also been confirmed in prostate cancer, liver cancer, bladder cancer and ovarian cancer [50]. Black garlic polysaccharides also have a certain immunomodulatory effect and have certain potential in adjuvant cancer treatment.

혈중 지질 저하 2.4

It has been reported that black garlic, when added to the daily regimen of patients suffering from stroke, heart disease, hypertension, etc., can lower blood lipids and cholesterol to a certain extent, and can be used as a daily health food. Yao Lu et al. [51] studied the lipid-lowering effect of black garlic and 검은 인삼 in combination and found that the blood lipid indicators of male rats fed a high-fat diet were significantly reduced, and the ginseng-garlic mixture has the potential to develop drugs for the prevention of hyperlipidemia. Black garlic can also improve the fatty degeneration around the portal triad and reduce fat accumulation in the liver. Research has shown that black garlic extract can inhibit lipogenesis and lipolysis in mature 3T3-L2 adipocytes by reducing the expression and phosphorylation of peroxisome proliferator-activated receptor gamma and the level of phospho-hormone sensitive lipase antibody, and regulates the basal metabolic activity of human adipose stem cells [52]. Park et al. [53] showed that black garlic extract reduced sterol regulatory element binding protein 1c mRNA expression, which in turn led to downregulation of lipid and cholesterol metabolism and a subsequent decrease in total blood lipids, triglycerides and cholesterol levels.

2.5기타 약리학적 효과

Black garlic has been reported to have a certain anti-allergic effect, but it is weaker than that of garlic. Yoo et al. [54] found that black garlic extract can inhibit immunoglobulin E-mediated allergic reactions in rat basophilic leukemia cells and passive skin allergic reactions in vivo. The mechanism of its anti-allergic effect involves the inhibition of tyrosine kinase, cytosolic phospholipase, arachidonate 5-lipoxygenase and COX-2. Black garlic extract can also significantly lower blood glucose and glycosylated hemoglobin, and significantly increase serum insulin. It has a beneficial effect on the biomarkers of glucose metabolism in rats with streptozotocin-induced diabetes.

The mechanism may be to increase the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and γ-glutamyl transferase, thereby lowering blood glucose [55]. In terms of liver protection, it can reduce the activity of alcohol-induced enzymes such as AST aminotransferase and ALT in the liver, inhibit the activity of cytochrome P450 2E2 and its induction of glutathione-s-transferase and quinone reductase activity, and exert a protective effect on chronic induced liver damage in SD rats [56]. Black garlic can regulate gastrointestinal motility, effectively stimulate gastrointestinal peristalsis by increasing serotonin content, enhance gastrointestinal emptying, and promote defecation [57]. Moon et al. [58] found through electrophysiological experiments that black garlic extract depolarizes the pacemaker potential of Cajal interstitial cells and also increases the small intestine propulsion rate of ICR mice. In addition, some studies have suggested that black garlic can be used to protect against nerve damage caused by fluorine poisoning [59].

3가공 기술

The black garlic processing technology in China was mainly introduced and improved from Japan, and mainly includes non-fermented processing technology and fermented processing technology. Among them, the non-fermented processing technology is more commonly used, and is divided into solid-state processing and liquid processing. Solid-state processing is currently the most important method for processing black garlic, which means that no other auxiliary materials are added, and the garlic is directly cultivated under the corresponding high temperature and high humidity conditions. The cultivation time has a great impact on the quality of black garlic, and the required accumulation of substances can only be achieved after more than 3 months. In order to shorten the processing time and ensure the quality, black garlic is often pre-processed before processing, including low-temperature freezing, high temperature, ultra-high pressure, microwave, and anaerobic respiration inhibition. Among them, low-temperature freezing and microwave can effectively shorten the processing time of black garlic and improve the functional components of black garlic. They are the most simple and efficient pre-processing methods. Zhang Xuehui et al. [60] also found that microwave-pretreated black garlic has higher levels of thiophene and furan substances, i.e., it has a richer charcoal-grilled aroma and fruity sweetness, with an even better flavor.

In recent years, research has tended to focus on the possibility of using microbial fermentation technology to increase the content of functional components in black garlic, which is greener and healthier than traditional processing methods. Many researchers believe that black garlic is a fermented product, and that microorganisms play a role in the formation of black garlic. Qiu Zhichang [61] showed through analysis of the endogenous bacterial community and characteristics that dominant endogenous bacteria can participate in the formation of black garlic, mainly by secreting fructan hydrolase to accelerate the Maillard reaction and promote browning of black garlic. It was also found that compared to the processing time, the Maillard reaction regulated by different bacteria has a greater impact on the quality of black garlic. However, further research is needed on how to use microbial fermentation to enhance the pharmacological activity of black garlic, the selection of fermentation strains, the determination and optimisation of fermentation processes, and safety issues.

4가지 제품 카테고리

4.1 흑마늘액 준비

Black garlic is often made into oral liquid. Its unique sweet and sour taste can mask the peculiar smell of other ingredients, making it more acceptable to patients. For example, black garlic can be used to improve the bitterness of Artemisia leaves extract and add flavour. Black garlic can be combined with many traditional Chinese medicines to prevent tumors, fight oxidation, lower blood lipids, and so on. In addition, black garlic can be made into black garlic enzyme oral liquid, black garlic fermented beverage, and paired with tea, Chinese medicinal materials, etc. to make “tea drinks”, and alcoholic products.

4.2 흑마늘 가루

Plant-based medicines or foods are often dried and ground to make powders, which have the characteristics of a long shelf life, being easy to digest and absorb, and being convenient to package and carry. Black garlic can be mixed and ground with the corresponding Chinese medicinal materials to make a compound nutritional powder, which can exert the effects of preventing and treating high blood pressure and hyperlipidemia, as well as being antioxidant. It is friendly in price and suitable for all kinds of people. The black garlic residue after allicin extraction can also be fermented and dried into black garlic residue powder. Fermentation increases the content of active ingredients in black garlic residue, which can not only be used as an additive in livestock feed, but also as an ingredient in various foods, making the best use of everything. At the same time, black garlic extract powder is also often used as a food additive to add flavor.

흑마늘 양념 4.3

연구자들은 또한 흑마늘을 피플 &에 도입하기 위해 노력하고 있다#39;s daily diets, making healthy seasonings such as black garlic vinegar, black garlic soy sauce, and black garlic hot sauce. Traditional soy sauce is a concentrated source of sodium (NaCl, monosodium glutamate, sodium benzoate), which is not friendly to people with hypertension, coronary heart disease, or diabetes. Black garlic soy sauce can avoid the health risks associated with high sodium content, satisfying consumers'맛있는 것과 건강에 대한 두 가지 욕구.이밖에 흑마늘식초에는 위를 보호하는 한약재가 들어있어 장기적으로 사용하면 위염, 위궤양 등 위질병을 효과적으로 완화시킬수 있다.

4.4기타 제품

Black garlic was first developed as a health food, but nowadays people prefer to develop it in a more functional direction. These include black garlic paste, which can moisten the bowels and promote digestion; black garlic jelly, which lowers the three highs; and black garlic candy, which moistens the bowels, prevents cancer and anti-aging. Black garlic can also be made into granules, such as black garlic maca granules, which have better fertility enhancement than maca granules alone. Black garlic soft capsules are a common form of black garlic medicine that more completely preserves the active ingredients of black garlic. In addition, black garlic has also begun to be used in skin care products, shampoos, and beauty products, attracting widespread attention.

5 전망

중국은 세계's largest garlic producer, with a production of 24.028 million tons in 2020, and the amount is increasing year by year. Export and further processing are the main ways of consuming garlic in China. Black garlic can greatly increase the added value of garlic and can be used as a new direction for the development of the garlic industry. Black garlic has been in China for less than 10 years, but it has quickly captured the market and is favored by consumers. It also has a large market in Japan, Singapore, Malaysia and other countries. Modern pharmacological research has proven that black garlic has a variety of pharmacological activities and has good development prospects. However, the current research is not highly refined, and people have not systematically understood the blackening mechanism and flavor formation rules of black garlic.

There has been less research on the components that affect the function of black garlic. Further in-depth research is conducive to the future application of black garlic-related foods and drug development, and is of great significance for increasing the added value of garlic and the economic benefits of enterprises. At present, there are many types of black garlic-related products, but there is a lack of brands with a large market share and a leading effect. In the future, it is necessary to vigorously promote the industrial development of black garlic, develop more specialty products, and seize the domestic and foreign markets. Combining chemical composition, pharmacological effects, and product categories, strengthening industry-university-research cooperation, using technology to lead industrial upgrading, and forming China's 특유의 흑마늘 산업.

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[10] 왕콩콩, 정젠지아, 루샤오밍 등.흑마늘 중 5-hydroxymethylfurfural의 형성법 및 안전성 평가 (윤정일, 정미숙, 정미숙)식품과학, 2022, 43(3):100-105.

[11] 양청리, 동안, 류유 외.흑마늘 가공 중 흑essence-related 성분의 성분 변화 및 성분 분석 [J.중국조미료, 2018, 43(9):73-77.

[12] 샤오케페이, 장기, 왕웨이 등.진향 신선마늘과 흑마늘의 영양성분 및 활성성분의 함량 비교 (J.중국식품첨가물, 2018, (6):141-144.

[13] 니우나나, 샤루이, 양첸밍 등.전처리 공정이 흑마늘의 기능성 성분 및 항산화 활성에 미치는 영향 및 관련 연구 [J.식품 및 발효산업, 2021, 47(8):67-75.

[14] 장쟁해, 동연, 지연루.흑마늘의 항산화 및 면역보호효과.중국조미료, 2022, 47(10):210-214.

[15] 선유 'e, Lv 다나, 왕위동 외.Maillard 반응이 마늘의 항산화 활성에 미치는 영향.식품산업과학기술원, 2013, (9):119-123.

[16] 모레노 O A, 디 P G, 페레이라 C G 등이 있다.In vitro colonic (체외 결장) (poly)phenols의 발효와 신선마늘과 흑마늘의 유기황화합물 (J. JAgric Food Chem, 2022, 70(12):3666-3677.

[17] 우팅, 류시시, 천중웨이 등.마이크로파-초음파 시너지 추출공정의 최적화 및 흑마늘 폴리페놀의 항산화 연구.전문연구, 2022, 44(4):81-89.

[18] 황자아, 양 자오,리 옌지 외.초음파를 이용한 흑마늘 폴리페놀의 추출과 in vitro 항산화 특성 [J.식품과학과 기술, 2018, 43(4):212-217.

[19] 송샤오홍, 류시치, 류잉잉 외.흑마늘 내 폴리페놀성 화합물의 마이크로파 보조 추출공정의 최적화 (J.산동농학, 2012, 44(5):110-113.

[20] 정청, 천바오인, 양레이 등.반응표면방법론에 의한 흑마늘로부터 폴리페놀과 플라보노이드를 동시 추출하는 공정의 최적화 및 항산화활성 측정에 대한 자료입니다.중국조미료, 2021, 46(5):69-76.

[21] 왕 Z,리 C,리 F 등.흑마늘 폴리페놀의 추출과 산법에 의한 항산화성에 관한 연구 (J.중국 식품 첨가물, 2021, 32(10):15-22.

[22] 유종명, 왕립신, 왕난니 등.복합효소를 이용한 반응표면법에 의한 흑마늘 폴리페놀 추출공정의 최적화에 관한 연구 (J.중국materia, 2018, 41(12):2883-2885.

[23] 노리안동, 주유, 황건적 등.동시의 결정의 내용을 알리, deoxynojirimycin 그리고 γ-glutamylcysteine 고성능 액체 크로마토그래피에 의해 검은 마늘다 [J다]동북농업대학 2020년도 51(7):36-43.

[24] Laskar A A, Danishuddin, Khan S H, et al. enhancement in the catalytic activity of human salivary aldehyde dehydrogenase by alliin from 마늘:implications in aldehyde toxicity and oral health[J].Curr Pharm Biotechnol, 2019, 20(6):506-516.

[25] 왕 W D, 선 Y E, 천 H W. 항산화제의 특성 흑마늘에 함유된 황화합물의 활성 (J.Cell Mol Biol, 2018, 64(12):76-80.

[26] Ahmed T, Wang C K. 흑마늘과 그 생리활성 화합물 on 인체건강질병:고찰 [J.분자, 2021, 26(16):5028.

[27] 호우옌린, 리톈자오, 리후이 외.흑마늘로부터 다당류의 초음파 보조 추출과 저혈당 효과에 관한 연구 (J.중국 과일과 채소, 2021, 41(9):53-58.

[28] 후먀오, 서홍지, 장쩌싱.x 선 방사선 손상을 입은 생쥐에 대한 흑마늘 다당류의 보호효과 [J.배화대학교 논문집 (자연과학편) 2021, 22(2):202-206.

[29] 장위안, 서빙신, 손위 등.흑마늘은 항산화 작용을 통해 쥐의 급성 알코올성 간 손상에 보호 효과를 발휘한다 [J].Strait Pharmacy, 2019, 31(10):24-27.

[30] 사요노 S, 나니 D, 프로베라와티아 외.검은 솔로 마늘이 보호해 준다 streptozotocin 유발 쥐에서 간세포 및 신세포 기능 (J.Front Nutr, 2022, 30(9):962993.

[31] 차이 J C, 첸 Y A, 우 J T, 그 외.발효된 흑의 추출물 마늘은 급성 간손상에 간보호 효과를 나타낸다 [J.분자, 2019, 24(6):1112.

[32] Kim H K. 고콜레스테롤혈증의 억제효과와 platelet in fermented and non-fermented preparation of 마늘 [J].Int J, 2019, 11:1-10.

[33] Zou Y, Zhao M, Yang K 외.대식성 수지를 이용한 흑마늘즙내 항산화제의 농축과 산화손상 인체적혈구에 대한 보호효과 (J.J Chromatogr B Analyt Technol Biomed Life Sci, 2017, 1060:443-450.

[34] 류제이, 장그, 콩 X 등.흑마늘은 순환 항산화 수준을 개선함으로써 관상 동맥 심장 질환 입원 환자의 심장 기능을 향상시킨다.Front Physiol, 2018, 9:1435.

[35] 발스 R M, 컴패니언즈 J, 칼데론 P L 외.an의 효과 심혈관 질환 위험에 최적화된 숙성 마늘 추출물 중도 고콜레스테롤혈증 대상자의 인자:무작위,

크로스 오버, 맹, sustainedand 통제 [J] 공부 한다.영양소, 2022, 14(3):405.

[36] Nurmasitoh T, Sari D C R, Partadiredja G. 흑마늘이 쥐의 내측 전전두피질의 작업기억과 피라미드세포 수에 미치는 영향 글루탐산나트륨 [J]에 노출됨.Drug Chem Toxicol, 2018, 41(3):324-329.

[37] Sun Y. 흑마늘의 제조 및 그 추출물이 생쥐의 학습 및 기억에 미치는 연구.광동:광동제약대학교, 2018.

[38] Chen C Y, Tsai T Y, Chen B H. 흑마늘 추출물과 nanoemulsion이 deoxy corticosterone acetate-salt 유발성 고혈압 및 이와 관련된 쥐의 경도인지장애에 미치는 영향 (J.산화방지제, 2021, 10(10):1611.

[39] 하aw, Kim W K. 핵인자인 erythroid 2-like factor 2 경로를 포함하는 흑마늘 추출물의 항산화 메커니즘 (J.Nutr Res Pract, 2017, 11(3):206-213.

[40] Lee T W, Bae E, Kim J H, 외.숙성된 흑의 수액 마늘은 쥐에서 콜리스틴으로 유발된 급성 신장손상을 개선한다 [J].Ren Fail, 2019, 41(1):24-33.

[41] 블라초자니스 C, 크루바식 H S, 헬위그 E 외.Spilanthes oleracea, propolis, Nigella sativa 및 흑마늘로부터 구강에 관여하는 다양한 미생물에 대한 조제의 활성 (Activity of preparations from Spilanthes oleracea, propolis, Nigella sativa, and black 마늘 dise.ases와 total human salivary bacteria:A pilot study[J]. 「 human salivary 」 (영어).Phytother Res, 2018, 32(10):1992-2001.

[42] 아이유 지유.항균 및 생체막 제거에 있어서 나노황화철의 기능 및 메커니즘 연구 [D.장쑤:양저우대학, 2019.

[43] 시룬동, 양천, 유자하오 외.생리 활성도가 높은 흑마늘의 가공기술 최적화 및 기능성 성분의 변화.한국식품생명공학회지, 2020, 39(12):71-79.

[44] Kim, K J, Kim, S H, Shin 외.S-allyl의 보호효과 시스틴계 농축 흑마늘이 nf-kb 신호경로를 통해 쥐의 역류성 식도염에 미치는 영향 (J.Funct Foods, 2019, 5:199-206.

[45] 최샤오유, 정홍영, 키잔원 외.궤양성 대장염 BALB/c 마우스에서 흑마늘이 염증반응에 미치는 영향 [J].식품과학, 2021, 42(7):185-190.

[46] Ashmawy N E, Khedr E G, Bahrawy H A 등.Gastroprotective indomethacin에서 마늘이 위궤양을 유발한 쥐에 미치는 영향 (J.Nutrition, 2016, 32:849-854.

[47] Shin D Y, Yoon M K, Choi Y W 외.숙성 흑마늘 추출물이 인체 위암세포에서 밀착접합투과성 및 세포침범에 미치는 영향 (Effects of the tight junction permeability and cell invasion in human gastric cancer cells)J 생명 Sci, 2010, 20(4):528-534.

[48] 뒤뤼슈.흑삼, 흑마늘 및 그 혼합물이 HepG-2세포의 사멸에 미치는 영향 [D].지린:옌볜대학, 2019.

[49] 숙성마늘 추출물의 Ohtani M, Nishimura T. sulfur 함유 아미노산은 세포간 유착분자-1발현과 IL-6분비를 억제함으로써 인체 치은상피세포의 염증을 억제한다 [J].Biol Rep, 2020, 12(3):99-108.

[50] 양기청, 왕동, 왕이산 외.루이스 폐암세포에 대한 흑마늘 추출물의 방사선 감작 효과 (J.대한한의학회지 (korean Journal of Integrated Traditional and Western Medicine) 2013, 33(8):1093-1097.

[51] 야오 L, 두 R, 바이 엑스 외.인삼과 마늘의 혼합물이 쥐의 고지혈증 예방 효과에 관한 관찰.중국수의학회지 2018, 54(8):94-96.

[52] Nam H, Jung H, Kim Y 외.숙성 흑마늘 추출물은 성숙한 3T3-L1 지방세포에서 지방생성을 억제하고 지방분해를 촉진함으로써 지질대사를 조절한다 [J].식품 Sci Biotechnol, 2018, 27:575-579.

[53] Park E, Baek S H, Bang K S 외.발효 마늘 추출물은 인체 지방 유래 줄기세포에서 산소 소비량과 UCP-1 mRNA 발현을 증가시킨다 [J.「 Cell J 」, 2019, 21(3):357-362.

[54] Yoo J M, Sok D E, Kim M R.의 RBL-2H3세포에서 숙성 흑마늘 추출물의 항알레르기 작용과 수동적 피부 anaphylaxis 반응에서 생쥐 (J.J Med Food, 2014, 17(1):92-102.

[55] 톰슨 M, 알 카타탄 K K, Js D 외.streptozotocin으로 유도된 당뇨쥐에서 숙성 마늘 추출물 (AGE)의 항당뇨 및 항산화 가능성 [J.BMC Complem Altern M, 2016, 19일: 16.

[56] Kim M H, Kim M J, Lee J H, 외.숙성 흑마늘의 만성 알코올로 유발된 쥐의 간 손상에 대한 간 보호 효과 (J.J 의약식품, 2011, 14(7-8):732-738.

[57] 체냐, 차이 J C, 청 K C 등.흑마늘의 추출물은 위장 운동 효과를 나타낸다 [J.Food ResInt, 2018, 107:102-109.

[58] Moon S B, Choi N R, Kim J N 외.흑마늘이 in vitro에서 murine 소장에서 카할의 간질세포의 pacemaker potentials에 미치는 영향과 in vivo에서 위장관 운동성에 미치는 영향 [J.Anim Cells Syst (서울), 2022, 26(1):37-44.

[59] 장이통, 장종링.불소 중독 신경 손상과 길항 보호에 대한 연구 진행 [J.Chinese Journal of Endemiology, 2019, 38(7):597-602.

[60] 장수휘, 류소오, 저카이경.전자레인지와 동결 전처리로 제조한 흑마늘의 감각 및 향미 품질 비교 분석.식품발효산업, 2022, 48(20):244-251.

[61] 아이유 지창.흑마늘 가공 중 미생물 군집의 구조 변화와 품질 형성에 미치는 영향 [D.산동:산동농업대학, 2022.